Journal: Cell Metabolism

Article Title: Nutrient-sensing AgRP neurons relay control of liver autophagy during energy deprivation

doi: 10.1016/j.cmet.2023.03.019

Figure Lengend Snippet:

Article Snippet: AAV8-GFP-U6-mNR3C1-shRNA , Vector Biolabs , Cat# shAAV-266053 RefSeq# NM_008173.

Techniques: Virus, Plasmid Preparation, Recombinant, Protease Inhibitor, Western Blot, Blocking Assay, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Isolation, Bicinchoninic Acid Protein Assay, Reverse Transcription, RNAscope, Multiplex Assay, Phospho-proteomics, Software, Microscopy, Mass Spectrometry, Liquid Chromatography, Chromatography

Journal: The Journal of Biological Chemistry

Article Title: An ANGPTL4–ceramide–protein kinase Cζ axis mediates chronic glucocorticoid exposure–induced hepatic steatosis and hypertriglyceridemia in mice

doi: 10.1074/jbc.RA118.006259

Figure Lengend Snippet: Myriocin (MYR) treatment reduced chronic dexamethasone exposure–induced hepatic steatosis and hypertriglyceridemia. WT and Angptl4−/− mice were treated with dexamethasone (0.84 mg/kg body weight) in drinking water for 7 days. Half of the mice received daily intraperitoneal injections of myriocin (0.5 mg/kg body weight) on days 4–7. A and B, at the end of treatment, the levels of TG in plasma (A) and liver (B) were measured. The error bars represent standard deviation (n = 6–8). *, p < 0.5. C, RNA from the liver of these mice was isolated, and the expression of genes involved in TG homeostasis was monitored by real-time PCR. The error bars represent S.E. (n = 7–9). *, p < 0.05. D, liver and epididymal white adipose tissue RNA was isolated, and gene expression for Angptl4 was measured. The error bars represent S.E. (n = 7–9). *, p < 0.05. WT mice were infected with adeno-associated virus (AAV8) expressing shRNA for scramble or Sptlc2 and were treated with dexamethasone for 2 weeks. E, liver was harvested and analyzed for decreased expression of Sptlc2. F and G, plasma (F) and liver (G) triglyceride levels were measured. The error bars represent standard deviation (n = 3–4). *, p < 0.05.

Article Snippet: AAV serotype 8 shRNA targeting Sptlc2 was purchased from Vector Biolabs.

Techniques: Clinical Proteomics, Standard Deviation, Isolation, Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Infection, Virus, shRNA